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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
05/05/2017 |
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Actualizado : |
24/06/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
OLINDA, R. G.; MAIA, L. A.; CARGNELUTTI, J. F.; GOIS, R. C. S.; BATISTA, J. S.; DANTAS, A. F. M.; FLORES, E. F.; RIET-CORREA, F. |
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Afiliación : |
FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.//Programa de Pós-Graduação, em Medicina Veterinária, Hospital Veterinário, Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande (UFCG), Brasil. |
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Título : |
Swinepox dermatitis in backyard pigs in Northeastern Brazil. |
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Fecha de publicación : |
2016 |
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Fuente / Imprenta : |
Pesquisa Veterinária Brasileira, Brasília, DF v. 36, n. 6, p. 468-472, 2016. |
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DOI : |
10.1590/S0100-736X2016000600002 |
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Idioma : |
Inglés |
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Notas : |
Article History:Received on June 11, 2015.//Accepted for publication on March 23, 2016. OPEN ACCESS |
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Contenido : |
This article describes five outbreaks of swinepox in backyard pigs in Northeastern Brazil. It affected backyard pigs from herds of poor hygienic-sanitary conditions with severe fly and lice infestations. The morbidity ranged from 33.3 to 100% among affected herds, with mortality reaching up to 60%. The affected pigs developed multifocal to coalescent gray to white papules and blisters in the skin, with eventual eruptions, evolving to erosions and crusts. In addition to skin lesions, affected piglets presented apathy, anorexia and fever. The disease was auto-limiting, resolving within 15 to 25 days. Histological examination revealed proliferative and ulcerative vesiculopustular dermatitis with ballooning degeneration of epithelial cells, perivascular inflammatory infiltrates of lymphocytes, plasma cells, neutrophils, eosinophils and some macrophages in the dermis. Intracytoplasmic eosinophilic inclusions were consistently observed in keratinocytes. Total DNA extracted from fresh tissue fragments obtained from one outbreak and formalin-fixed, paraffin-embedded (FFPE) tissue from the other four outbreaks was submitted to polymerase chain reaction (PCR) for Swinepox virus (SWPV) and Vaccinia virus (VACV). Genetic SWPV material was identified by PCR in fresh material from one outbreak. Nucleotide sequencing and phylogenetic analysis of the PCR amplicons (viral polymerase gene) demonstrated 100% homology with sequences from SWPV. All tissues were PCR negative for VACV. Swine poxvirus is present in backyard pigs in Northeastern Brazil, indicating the need of including SWPV in the differential diagnosis of dermatitis in pigs. MenosThis article describes five outbreaks of swinepox in backyard pigs in Northeastern Brazil. It affected backyard pigs from herds of poor hygienic-sanitary conditions with severe fly and lice infestations. The morbidity ranged from 33.3 to 100% among affected herds, with mortality reaching up to 60%. The affected pigs developed multifocal to coalescent gray to white papules and blisters in the skin, with eventual eruptions, evolving to erosions and crusts. In addition to skin lesions, affected piglets presented apathy, anorexia and fever. The disease was auto-limiting, resolving within 15 to 25 days. Histological examination revealed proliferative and ulcerative vesiculopustular dermatitis with ballooning degeneration of epithelial cells, perivascular inflammatory infiltrates of lymphocytes, plasma cells, neutrophils, eosinophils and some macrophages in the dermis. Intracytoplasmic eosinophilic inclusions were consistently observed in keratinocytes. Total DNA extracted from fresh tissue fragments obtained from one outbreak and formalin-fixed, paraffin-embedded (FFPE) tissue from the other four outbreaks was submitted to polymerase chain reaction (PCR) for Swinepox virus (SWPV) and Vaccinia virus (VACV). Genetic SWPV material was identified by PCR in fresh material from one outbreak. Nucleotide sequencing and phylogenetic analysis of the PCR amplicons (viral polymerase gene) demonstrated 100% homology with sequences from SWPV. All tissues were PCR negative for VACV. Swine poxvi... Presentar Todo |
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Palabras claves : |
Doença viral. |
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Thesagro : |
Dermatite; Dermatitis; Poxviridae; Suíno; Swine; Varíola; Viral diseases of animals and humans. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/6764/1/Pesq.-Vet.-Bras.-366p.468-472-2016.pdf
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Marc : |
LEADER 02654naa a2200325 a 4500
001 1057176
005 2021-06-24
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1590/S0100-736X2016000600002$2DOI
100 1 $aOLINDA, R. G.
245 $aSwinepox dermatitis in backyard pigs in Northeastern Brazil.$h[electronic resource]
260 $c2016
500 $aArticle History:Received on June 11, 2015.//Accepted for publication on March 23, 2016. OPEN ACCESS
520 $aThis article describes five outbreaks of swinepox in backyard pigs in Northeastern Brazil. It affected backyard pigs from herds of poor hygienic-sanitary conditions with severe fly and lice infestations. The morbidity ranged from 33.3 to 100% among affected herds, with mortality reaching up to 60%. The affected pigs developed multifocal to coalescent gray to white papules and blisters in the skin, with eventual eruptions, evolving to erosions and crusts. In addition to skin lesions, affected piglets presented apathy, anorexia and fever. The disease was auto-limiting, resolving within 15 to 25 days. Histological examination revealed proliferative and ulcerative vesiculopustular dermatitis with ballooning degeneration of epithelial cells, perivascular inflammatory infiltrates of lymphocytes, plasma cells, neutrophils, eosinophils and some macrophages in the dermis. Intracytoplasmic eosinophilic inclusions were consistently observed in keratinocytes. Total DNA extracted from fresh tissue fragments obtained from one outbreak and formalin-fixed, paraffin-embedded (FFPE) tissue from the other four outbreaks was submitted to polymerase chain reaction (PCR) for Swinepox virus (SWPV) and Vaccinia virus (VACV). Genetic SWPV material was identified by PCR in fresh material from one outbreak. Nucleotide sequencing and phylogenetic analysis of the PCR amplicons (viral polymerase gene) demonstrated 100% homology with sequences from SWPV. All tissues were PCR negative for VACV. Swine poxvirus is present in backyard pigs in Northeastern Brazil, indicating the need of including SWPV in the differential diagnosis of dermatitis in pigs.
650 $aDermatite
650 $aDermatitis
650 $aPoxviridae
650 $aSuíno
650 $aSwine
650 $aVaríola
650 $aViral diseases of animals and humans
653 $aDoença viral
700 1 $aMAIA, L. A.
700 1 $aCARGNELUTTI, J. F.
700 1 $aGOIS, R. C. S.
700 1 $aBATISTA, J. S.
700 1 $aDANTAS, A. F. M.
700 1 $aFLORES, E. F.
700 1 $aRIET-CORREA, F.
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 36, n. 6, p. 468-472, 2016.
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Registro original : |
INIA La Estanzuela (LE) |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Las Brujas. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
04/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
B - 2 |
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Autor : |
LADO, J.; MANZI, M.; SILVA, G.; LUQUE, E.; BLANCO, O.; PEREZ, E. |
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Afiliación : |
JOANNA LADO LINDNER, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MATIAS JESUS MANZI FRAGA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; GUILLERMO DANIEL SILVA MAINARDI, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MAYZA ELEANA LUQUE NUÑEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORIBE BLANCO MARTINEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; ELENA PEREZ FAGGIANI, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Effective alternatives for the postharvest control of imazalil resistant Penicillium digitatum strains. |
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Complemento del título : |
Conference Paper. |
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Fecha de publicación : |
2010 |
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Fuente / Imprenta : |
Acta Horticulturae, 2010, no. 877, p.1449-1456. |
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ISBN : |
978-906605613-8 |
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ISSN : |
0567-7572 |
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DOI : |
10.17660/ActaHortic.2010.877.198 |
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Idioma : |
Inglés |
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Notas : |
ISHS Acta Horticulturae 877: VI International Postharvest Symposium. |
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Contenido : |
ABSTRACT.
Postharvest diseases control in Citrus is essential to maintain fruit quality during storage, overseas transport and selling. Green mold caused by Penicillium digitatum is one of the most important postharvest diseases. In Uruguay it has been controlled by synthetic fungicides. Fungicide resistant strains make it neccesary to develop new strategies to control postharvest decay in Citrus. In view of this situation, we attempted to develop an effective alternative management to control Penicillium digitatum imazalil (IMZ) resistant strains. For this purpose 'Valencia' oranges were inoculated with imazalil sensitive SS-22 and resistant RS-20 strains selected previously according to their aggressiveness on lemon fruits. From 18-22 hours before treatments, each fruit was inoculated by wounding (depth 2 mm, breadth 1 mm) and inoculating with a P. digitatum spore suspension (1,0 × 106 spores/ml). Incidence and sporulation was evaluated after 7, 11 and 15 days at 20°C. Similarly, an experimental packing-line was used to evaluate chemical alternatives and application methods. Imazalil with pyrimethanil (Philabuster"-PI, 750 ppm) mixture or combined with potassium sorbate (KS, 2%) resulted in a significant control of the incidence and sporulation of the most aggressive P. digitatum resistant strain. The incidence was reduced from 75-80% in controls to 0-8% in treatments. IMZ applied at 1500 ppm did not control the incidence or sporulation of the resistant strain. The application of KS reduced strains incidence and sporulation by 50-70%. No difference (p?0.05) was found in the pathogen control comparing both application methods. All treatments were effective on reducing sensible strain sporulation, being only PI alone or combined with KS, effective for the resistant strain. We concluded that PI (750 ppm) alone or with KS at 2% can be applied as an alternative management to control Uruguayan Penicillium digitatum sensitive and resistant IMZ strains. MenosABSTRACT.
Postharvest diseases control in Citrus is essential to maintain fruit quality during storage, overseas transport and selling. Green mold caused by Penicillium digitatum is one of the most important postharvest diseases. In Uruguay it has been controlled by synthetic fungicides. Fungicide resistant strains make it neccesary to develop new strategies to control postharvest decay in Citrus. In view of this situation, we attempted to develop an effective alternative management to control Penicillium digitatum imazalil (IMZ) resistant strains. For this purpose 'Valencia' oranges were inoculated with imazalil sensitive SS-22 and resistant RS-20 strains selected previously according to their aggressiveness on lemon fruits. From 18-22 hours before treatments, each fruit was inoculated by wounding (depth 2 mm, breadth 1 mm) and inoculating with a P. digitatum spore suspension (1,0 × 106 spores/ml). Incidence and sporulation was evaluated after 7, 11 and 15 days at 20°C. Similarly, an experimental packing-line was used to evaluate chemical alternatives and application methods. Imazalil with pyrimethanil (Philabuster"-PI, 750 ppm) mixture or combined with potassium sorbate (KS, 2%) resulted in a significant control of the incidence and sporulation of the most aggressive P. digitatum resistant strain. The incidence was reduced from 75-80% in controls to 0-8% in treatments. IMZ applied at 1500 ppm did not control the incidence or sporulation of the resistant strain. The applic... Presentar Todo |
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Palabras claves : |
Philabuster; Potassium sorbate; Pyrimethanil. |
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Thesagro : |
BACTERIOSIS; CITRUS; ENFERMEDADES DE LAS PLANTAS. |
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Asunto categoría : |
--
H20 Enfermedades de las plantas |
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Marc : |
LEADER 02867naa a2200301 a 4500
001 1012844
005 2019-10-04
008 2010 bl uuuu u00u1 u #d
020 $a978-906605613-8
022 $a0567-7572
024 7 $a10.17660/ActaHortic.2010.877.198$2DOI
100 1 $aLADO, J.
245 $aEffective alternatives for the postharvest control of imazalil resistant Penicillium digitatum strains.
260 $c2010
500 $aISHS Acta Horticulturae 877: VI International Postharvest Symposium.
520 $aABSTRACT. Postharvest diseases control in Citrus is essential to maintain fruit quality during storage, overseas transport and selling. Green mold caused by Penicillium digitatum is one of the most important postharvest diseases. In Uruguay it has been controlled by synthetic fungicides. Fungicide resistant strains make it neccesary to develop new strategies to control postharvest decay in Citrus. In view of this situation, we attempted to develop an effective alternative management to control Penicillium digitatum imazalil (IMZ) resistant strains. For this purpose 'Valencia' oranges were inoculated with imazalil sensitive SS-22 and resistant RS-20 strains selected previously according to their aggressiveness on lemon fruits. From 18-22 hours before treatments, each fruit was inoculated by wounding (depth 2 mm, breadth 1 mm) and inoculating with a P. digitatum spore suspension (1,0 × 106 spores/ml). Incidence and sporulation was evaluated after 7, 11 and 15 days at 20°C. Similarly, an experimental packing-line was used to evaluate chemical alternatives and application methods. Imazalil with pyrimethanil (Philabuster"-PI, 750 ppm) mixture or combined with potassium sorbate (KS, 2%) resulted in a significant control of the incidence and sporulation of the most aggressive P. digitatum resistant strain. The incidence was reduced from 75-80% in controls to 0-8% in treatments. IMZ applied at 1500 ppm did not control the incidence or sporulation of the resistant strain. The application of KS reduced strains incidence and sporulation by 50-70%. No difference (p?0.05) was found in the pathogen control comparing both application methods. All treatments were effective on reducing sensible strain sporulation, being only PI alone or combined with KS, effective for the resistant strain. We concluded that PI (750 ppm) alone or with KS at 2% can be applied as an alternative management to control Uruguayan Penicillium digitatum sensitive and resistant IMZ strains.
650 $aBACTERIOSIS
650 $aCITRUS
650 $aENFERMEDADES DE LAS PLANTAS
653 $aPhilabuster
653 $aPotassium sorbate
653 $aPyrimethanil
700 1 $aMANZI, M.
700 1 $aSILVA, G.
700 1 $aLUQUE, E.
700 1 $aBLANCO, O.
700 1 $aPEREZ, E.
773 $tActa Horticulturae, 2010, no. 877, p.1449-1456.
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